Clinical observation on acupoint injection of Chuankezhi injection plus acupoint sticking in treating bronchial asthma / 针灸推拿医学（英文版）

Objective: To observe the clinical efficacy of acupoint injection of Chuankezhi plus acupoint sticking treatment for bronchial asthma, and provide clinical evidence for the synergy of the two therapies, and explore their synergistic mechanism. Methods: A total of 70 patients were randomized into an acupoint injection plus sticking group and an acupiont sticking group by the random number table, with 35 cases in each group. The treatment took place in July and August. The acupoint injection plus sticking group was treated with acupoint injection and acupiont sticking, while the acupiont sticking group was treated only with acupiont sticking therapy. The treatment course was 4 weeks. After the treatment, the scores of symptom scale in the two groups before treatment, 3 months and 6 months after the treatment were observed. Results: During the treatment, there were 5 dropouts in the acupoint injection plus sticking group with 30 cases remained, and 4 dropouts in the acupiont sticking group with 31 cases remained. Before the treatment, there was no significant difference in the total scores of symptom scale between the two groups. Three months after the treatment, the total scores of symptom scale of both groups were lower than those before treatment, and the intra-group differences were statistically significant (bothP<0.05). In the inter-group comparison, there was no significant difference in the difference values of the scores before treatment and 3 months after treatment (P>0.05). Six months after the treatment, the total scores of symptom scale in both groups were lower than those before treatment, and the intra-group differences were statistically significant (bothP<0.05), so were the differences in the difference values of the scores before treatment and 6 months after treatment (bothP<0.05). Conclusion: Acupoint injection of Chuankezhi plus acupoint sticking or acupoint sticking alone both can improve the symptoms of patients with bronchial asthma. Acupoint injection of Chuankezhi plus acupoint sticking has a higher total effective rate than single acupoint sticking therapy.

Study on the relationship of serum vitamin E and liver pathological features in the patients with chronic hepatitis B / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To study on the relationship of serum vitamin E and liver pathological features in the patients with chronic hepatitis B.</p><p><b>METHODS</b>Sixty-six patients with chronic hepatitis B and ten healthy controls were enrolled in this present study. The serum vitamin E level was measured spectrophotometrically. Comparisons of liver function test, HBeAg and HBV DNA level were conducted among different liver pathological features including inflammatory grading and fibrosis staging.</p><p><b>RESULTS</b>Compared with healthy controls, the serum level of vitamin E was significantly decreased in the patients with chronic hepatitis B, especially in those with elevated ALT activity. In comparison between HBeAg positive group and HBeAg negative group, the serum level of vitamin E of the former group did not significantly changed (P > 0.05). Furthermore, the serum level of vitamin E has been demonstrated to be negatively associated with the inflammation grading in the patients with chronic hepatitis B. However, there was no significant association between the serum vitamin E and liver fibrosis staging.</p><p><b>CONCLUSION</b>Vitamin E, as one of the important anti-oxidants, was demonstrated to be implicated in the progression of liver inflammation in the patients with chronic hepatitis B. Furthermore, the supplement of vitamin E would be a potential therapy for attenuate the inflammatory response.</p>

Study of serum level of cortisol and peripheral T lymphocyte subsets state in the hepatitis B virus carriers / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To study of serum level of cortisol and peripheral T lymphocyte subsets state in the hepatitis B virus (HBV) carriers.</p><p><b>METHODS</b>Sixty chronic HBV carriers and ten healthy controls were all enrolled in this present study. Serum expression of cortisol was determined by radioimmunoassay, and also flow cytometry was performed to evaluate peripheral blood T lymphocyte subset.</p><p><b>RESULTS</b>Compared with those in normal controls, the serous levels of cortisol in chronic HBV carriers were significantly elevated, while there was no distinct difference in the proportion of CD4+ T lymphocytes ( P > 0.05) with the decreased odds of CD4+/CD8+ lymphocytes( P < 0.05) and obvious higher proportion of CD8+ T lymphocytes( P < 0.05). In comparison between HBeAg positive group and HBeAg negative group, the serous levels of cortisol of the former group were significantly higher ( P < 0.05), and so proportion of CD8+ T was too ( P < 0.05). However, there is no significant differences in the proportion of CD4+ T lymphocyte ( P > 0.05).</p><p><b>CONCLUSION</b>The elevated serum cortisol and increased CD8+ T lymphocytes subsets in the chronic HBV carriers, suggested that there was disturbance of endocrine-immune response in the chronicity of HBV infection.</p>

Safety and tolerance study of single oral dose of adefovir dipivoxil tablets in healthy volunteers / 中华实验和临床病毒学杂志

<p><b>BACKGROUND</b>To assess the safety and tolerance of adefovir dipivoxil tablet in Chinese healthy volunteers.</p><p><b>METHODS</b>Totally 42 healthy volunteers were enrolled in the study, 21 were female and 21 were male and their age ranged from 19 to 26 years. The subjects were randomly divided into 5, 10, 20, 40 and 60 mg dose-groups (6-10 subjects in each group) based on sex and weight, beginning with the 5 mg dose-group. Clinical symptoms, vital signs, electrocardiogram, routine blood test, routine urine test, prothrombin time and blood biochemical tests were recorded and evaluated.</p><p><b>RESULTS</b>No significant changes were found in clinical symptoms, vital signs and laboratory tests after dosing, except slight elevations of alanine aminotransferase in 2 subjects and bilirubin in 6 subjects observed and some gastrointestinal symptoms such as nausea and diarrhea found in 3 subjects, but the frequency and severity of all the adverse reactions were not found to be related to the dosages.</p><p><b>CONCLUSION</b>The results showed that single oral dose of adefovir dipivoxil 60 mg or less was safe and tolerable.</p>

Oxidative stress in patients with chronic hepatitis B / 中华实验和临床病毒学杂志

<p><b>BACKGROUND</b>To study oxidative stress in patients with chronic hepatitis B.</p><p><b>METHODS</b>Malondialdehyde (MDA), total anti-oxidative ability and ascorbic acid were measured as markers of oxidative stress in 30 patients with chronic hepatitis B, besides HBV DNA and ALT.</p><p><b>RESULTS</b>MDA was significantly higher in patients with hepatitis B than the controls (P less than 0.05). Ascorbic acid was significantly higher in patients with normal ALT than the controls (P less than 0.01). MDA was significantly higher in patients with increased ALT than the controls and in patients with normal ALT. MDA was significantly positively correlated with ALT (r=0.61), and ascorbic acid was significantly negatively correlated with ALT (r=-0.64) in patients with hepatitis B. No significant relationship was found between HBV DNA and other indices of oxidative stress. No significant difference in total anti-oxidative ability was found among all groups.</p><p><b>CONCLUSION</b>There was a disturbance between oxidative stress and anti-oxidative ability in patients with chronic hepatitis B. In patients with increased ALT, oxidative stress became high. In patients with normal ALT, oxidative stress level was low. The indices of oxidative stress should be detected in patients with hepatitis B, in addition to HBV markers.</p>

Hemorheologic changes in patients with liver diseases / 中华实验和临床病毒学杂志

<p><b>BACKGROUND</b>To study the variation of the hemorheology and microcirculation in different period of liver diseases.</p><p><b>METHODS</b>Indices for hemorheology, liver function, HBV DNA and transfusion transmitted virus (TTV) DNA were measured in 82 patients with liver diseases and correlative analysis was made.</p><p><b>RESULTS</b>The low-shear whole blood viscosity (BV) and RBC aggregation index were significantly higher in hepatitis B group than those in the control group (P less than 0.05). No correlation was found between HBV DNA and indices of hemorheology (P greater than 0.05). The high-shear and low-shear BV and hematocrit (HCT) were significantly lower in decompensated cirrhosis group than those in the control group (P less than 0.05). RBC aggregation index, plasma viscosity (PV) and the low-shear BV were significantly higher in compensated cirrhosis group than those in the control group (P less than 0.05). The high-shear and low-shear BV were significantly higher in TTV positive group than those in the control group.</p><p><b>CONCLUSION</b>There is disturbance of microcirculation in the body of patients with hepatitis B or TTV infection. The blood of patients with compensated cirrhosis is in highly viscose status and in low viscose status in patients with decompensated cirrhosis. TTV seems to be harmful to some degree to the body. The hemorheology should be an index in detecting liver diseases in addition to HBV markers.</p>